We are excited to announce funding by EMBO for implementing a workshop on circular RNAs.
Organizers: Vladimir Benes (main), Irene Bozzoni, Marie-Laure Baudet and
Category: EMBO Practical Course
Title: EMBO Practical Course: Methods for analysis of circular RNAs: No tautology
Dates: 17 November 2019 – 22 November 2019
Join us for an advanced training experience using the Oxford Nanopore Technologies (ONT) platform. We will start with an introduction into ONT technology and devices, with the goal of covering end to end workflows for the preparation and analysis of human and yeast samples using whole genome and barcoded cDNA sequencing approaches. This course will cover the wet lab preparation of libraries from genomic DNA and total RNA, with a focus on the critical steps and potential pitfalls and understanding what constitutes a ‘good’ sample for purpose of best results using the technology. The training includes an overview of the MinKNOW GUI for GridION and MinION devices. We then cover methods available for basecalling and analysis of samples for structural variants and differential gene expression, using both Oxford Nanopore and open source tools.
More information and application at https://www.embl.de/training/events/2019/NAN19-01/index.html
Our software contribution
FUCHS – Towards full circular RNA characterization using RNAseq
has been selected for a talk at GCB2016
You will find a preprint at https://peerj.com/preprints/2418/